ROCK1 mediates leukocyte recruitment and neointima formation following vascular injury
J. Clin. Invest. Kensuke Noma, et al. 118:1632 doi:10.1172/JCI29226 [
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Figure 5Decreased expression of endothelial adhesion molecules in
Rock1+/– mice.
Representative histological sections from unligated and ligated carotid arteries at 7 days after ligation in WT and
Rock1+/– mice stained for ICAM-1 (
A) and VCAM-1 (
B). Scale bars: 50 μm. (
C) Representative result of northern blot analysis of
Icam1 and
Vcam1 mRNA expression in ECs from WT and
Rock1+/– mice. ECs were stimulated with 5 U/ml of thrombin for 6 hours.
Gapdh mRNA expression was used as an internal control. (
D) Representative result of western blot analysis of ROCK1 and ROCK2 expressions in ECs from WT and
Rock1+/– mice with or without thrombin stimulation for 5 minutes. Actin was used as an internal control. (
E) Representative result of western blot analysis of ROCK and ERK activities in ECs from WT and
Rock1+/– mice. ECs were stimulated with 5 U/ml of thrombin for the indicated time periods.
