p21Cip1 modulates arterial wound repair through the stromal cell–derived factor-1/CXCR4 axis in mice
J. Clin. Invest. Michelle Olive, et al. 118:2050 doi:10.1172/JCI31244 [
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Figure 7SDF-1 inhibition prevents excessive proliferation during vascular wound repair. (
A) Reduced vascular lesions in
p21–/– (left) and
p21+/+ arteries (right) after AMD3100 treatment compared with saline-treated animals (Co) 1 and 2 weeks after vascular injury (left,
n = 5; right,
n = 10). (
B) Reduced vascular lesions in
p21–/– and
p21+/+ arteries with anti-CXCR4–blocking antibody compared with mice treated with IgG control (left,
n = 5; right,
n = 16). (
C) Anti-CXCR4 blocking antibody decreased cellular proliferation as assessed by BrdU incorporation at 1 and 2 weeks after vascular injury in
p21–/– and
p21+/+ arteries (left,
n = 5; right,
n = 5). (
D) Anti-CXCR4 blocking antibody reduced the number of local arterial macrophages in
p21–/– and
p21+/+ arteries at 1 and 2 weeks after vascular injury (left,
n = 5; right,
n = 5). (
E) The number of local apoptotic TUNEL-positive cells after vascular injury was unchanged after treatment with anti-CXCR4 blocking antibody. *
P < 0.05, **
P < 0.01, and ***
P < 0.001 versus Co at same time point.
