Ablation of GalNAc-4-sulfotransferase-1 enhances reproduction by altering the carbohydrate structures of luteinizing hormone in mice
J. Clin. Invest. Yiling Mi, et al. 118:1815 doi:10.1172/JCI32467 [Go to this article.]

Figure 8
GalNAc-4-ST1^–/– mice do not add sulfate to N-linked carbohydrates on the glycoprotein hormones. Extracts prepared from 5 WT and 5 GalNAc-4-ST1^–/– mice that had not been castrated (A and C, left) or that had been castrated (B and C, right) were separated by SDS-PAGE and electrophoretically transferred to PVDF membranes for Western blot analysis. (A) Cys-Fc (chimeric protein specific for terminal GalNAc-4-SO₄). (B) Mab6.3 (monoclonal antibody specific for SO₄-4-GalNAcβ1,4GlcNAcβ1,2Man-). (C) Rabbit anti-LHβ. The location of the bands identified as the α subunit (not shown) and the LHβ subunit (C) in separate blots are indicated.