A novel antiplatelet antibody therapy that induces cAMP-dependent endocytosis of the GPVI/Fc receptor γ-chain complex
J. Clin. Invest. Hiroshi Takayama, et al. 118:1785 doi:10.1172/JCI32513 [Go to this article.]

Figure 4
Effects of mF1232 or mF1201 injected into cynomolgus monkeys on GPVI immunodepletion, collagen-induced platelet aggregation, and platelet counts. mF1232 (white bars) or mF1201 (black bars) was i.v. injected at a dose of 0.3 mg/kg into monkeys. Blood was collected on day 0 (0d), day 1 (1d), and day 2 (2d) after injection. The level of GPVI, FcRγ, and GPIIIa in platelet lysates (A) and the expression of surface GPVI (B) were analyzed by western blotting and by flow cytometry, respectively, using each specific Ab. (A) Representative data of western blotting from 3 separate experiments are shown at the top of the panel. The band density of GPVI (middle panel) or FcRγ (bottom panel) was quantified by a Typhoon Scanner 9410 and shown as percentage of that on day 0. (B) Mean fluorescence intensity was normalized to 100 for that on day 0. Maximal platelet aggregation was monitored using PRP stimulated with 2 μg/ml collagen (C), and platelet (PLT) counts in whole blood (D) were determined. Data are presented as mean ± SEM of 3 separate experiments. *P < 0.05, **P < 0.01 versus day 0.