A novel antiplatelet antibody therapy that induces cAMP-dependent endocytosis of the GPVI/Fc receptor γ-chain complex
J. Clin. Invest. Hiroshi Takayama, et al. 118:1785 doi:10.1172/JCI32513 [Go to this article.]

Figure 5
CypHer5E-labeled cF1232 injected into cynomolgus monkeys is endocytosed into circulating platelets correlating with GPVI depletion. CypHer5E-labeled cF1232 or human IgG was i.v. injected at a dose of 1 mg/kg into cynomolgus monkeys. The fluorescence intensity (A) in circulating platelets and platelet surface GPVI expression (B) at the indicated time points after injection of CypHer-5E–labeled cF1232 (open circles) or human IgG (open triangles) were analyzed by flow cytometry. The levels of surface GPVI expression are normalized to 100 for day 0. The filled circles with dotted lines represent monkeys pretreated with 1 mg/kg unlabeled cF1232. GPVI and FcRγ expression in circulating platelets at the indicated time points after injection were analyzed by western blotting (C). All data are presented as mean ± SEM of 3 separate experiments. *P < 0.05, **P < 0.01 versus control groups at the same time points.