Adiponectin regulates albuminuria and podocyte function in mice
J. Clin. Invest. Kumar Sharma, et al. 118:1645 doi:10.1172/JCI32691 [Go to this article.]

Figure 2
Ad^–/– mice exhibit increased albuminuria, oxidant stress, and podocyte dysfunction. (A) Urine ACR in Ad^–/– mice significantly increased compared with corresponding age-matched WT mice at 1, 2, 3, and 4 months of age (n = 10 per group). *P < 0.01 versus corresponding age-matched WT. (B) WT and Ad^–/– mice were made diabetic with low-dose streptozotocin, and urine ACR was measured before and at 2 and 4 months of diabetes. Albuminuria was significantly increased in Ad^–/– mice with diabetes compared with corresponding WT diabetic groups (n = 5–10 per group). DM, diabetes mellitus. *P < 0.05 versus WT control; **P < 0.05 versus WT DM at 2 mo; ***P < 0.05 versus WT DM at 4 mo. (C) Urinary hydrogen peroxide/creatinine levels significantly increased in Ad^–/– mice with and without diabetes (n = 10 per group). *P < 0.05 versus WT control; **P < 0.05 versus WT DM at 2 mo. Values are mean ± SEM. (D) Podocyte foot processes were segmentally effaced in Ad^–/– mouse kidneys by EM. Arrows denote areas of normal foot processes in WT kidneys and areas of foot process effacement in Ad^–/– glomeruli. Images are representative of 10 EM images per kidney from 2 mice per group. Original magnification, ×5,000.