In vivo imaging in mice reveals local cell dynamics and inflammation in obese adipose tissue
J. Clin. Invest. Satoshi Nishimura, et al. 118:710 doi:10.1172/JCI33328 [
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Figure 1Schematic diagram of the confocal microscope for real-time in vivo imaging. To visualize cell dynamics in vivo, fluorescent dyes were injected into the tail veins of anesthetized mice. A small incision was then made for an observation window, and the mouse was set on a heating pad to maintain body temperature. An inverted microscope equipped for Nipkow–spinning disk confocal laser microscopy, which enabled scanning at up to 1,000 frames/s, was used to visualize the tissue. See Methods for details.
