In vivo imaging in mice reveals local cell dynamics and inflammation in obese adipose tissue
J. Clin. Invest. Satoshi Nishimura, et al. 118:710 doi:10.1172/JCI33328 [Go to this article.]

Figure 4
Enhanced surface expression of adhesion molecules in macrophages and ECs in obese adipose tissue. (A–C) Adhesion molecule expression examined by real-time PCR. mRNA expression of P- and E-selectin and ICAM-1 in the SVF of adipose tissue from ob/+ and ob/ob mice was analyzed (n = 5). (D–L) FACS analysis of the surface expression of adhesion molecules in F4/80⁺ macrophages (D–H) and CD31⁺ ECs (I–L) from ob/+ (gray line) and ob/ob (black line) mice. Expression of ICAM-1, L-selectin, and CD11c was upregulated in ob/ob compared with ob/+ macrophages. E-selectin, P-selectin, and ICAM-1 expression was upregulated in ob/ob ECs compared with ob/+ ECs. (M) Expression of ICAM-1 in F4/80⁺ cells was examined in local adipose blood. The y axis denotes cell count; the x axis denotes signal intensity. *P < 0.05.