In vivo imaging in mice reveals local cell dynamics and inflammation in obese adipose tissue
J. Clin. Invest. Satoshi Nishimura, et al. 118:710 doi:10.1172/JCI33328 [Go to this article.]

Figure 5
Increased platelet P-selectin expression and formation of monocyte-platelet conjugates in obese adipose tissue. (A and B) Platelet surface P-selectin expression in systemic circulation (A) and local adipose blood (B) from ob/+ (gray line) and ob/ob (black line) mice was examined to evaluate the activation status of platelets. Platelets were gated based on CD41 expression and side scatter. Platelet activation was indicated in obese adipose tissue blood, but not in systemic blood from ob/ob mice. The y axis denotes cell count; the x axis denotes signal intensity. (C) Quantification of monocyte-platelet conjugates in the systemic circulation and adipose tissue blood. Systemic and local blood obtained from adipose tissue was examined by FACS using F4/80 (macrophage) and CD41 (platelet) gates. The ratio of the F4/80⁺CD41⁺ cell (conjugates) to the total F4/80⁺ cell count (macrophages) is shown (n = 5 per genotype). *P < 0.05.