Bim-mediated deletion of antigen-specific CD8⁺ T cells in patients unable to control HBV infection
J. Clin. Invest. A. Ross Lopes, et al. 118:1835 doi:10.1172/JCI33402 [Go to this article.]

Figure 1
cDNA microarray data of HBV-specific CD8⁺ T cells from resolved and CHB patients. (A) Envelope-specific CD8⁺ T cells were purified by flow cytometric sorting of a short-term PBMC line from a chronic patient (middle and left plots, respectively). mRNA extracted from the purified cells was then profiled by dual-color cDNA microarray technology (right). (B) TreeView analysis of average linkage hierarchically clustered (with self-organized mapping) gene expression data. The top dendrogram represents the similarity between individual arrayed samples (vertical plane) based on the global gene expression profile; a yellow line segregates the main clusters: chronic (C) and resolved (R). The blue box highlights a section of the heat map where a group of genes exhibited marked upregulation in branch C compared with R (listed with original and current unigene references on the right; genes in red participate in apoptosis; genes overlapping with the SAM short list are indicated by asterisks). (C) SAM plot illustrating the most significant differentially regulated genes (false discovery rate, 1.3%) between the group with chronic and that with resolved HBV infection. (D) cDNA array data of 5 highly significant apoptosis-related genes. Error bars indicate mean ± SD.