Dopamine 5 receptor mediates Ang II type 1 receptor degradation via a ubiquitin-proteasome pathway in mice and human cells
J. Clin. Invest. Hewang Li, et al. 118:2180 doi:10.1172/JCI33637 [Go to this article.]

Figure 1
Effect of the AT₁R antagonist losartan on blood pressure and AT₁R and renin protein expression in Drd5^+/+ and Drd5^–/– mice. Vehicle (Veh) or losartan (Los) was administered via intraperitoneal injection every day for 5–7 days in 6-month-old mice. (A) Blood pressure was measured directly via the femoral artery under pentobarbital anesthesia. Losartan decreased blood pressure in Drd5^–/– but not Drd5^+/+ mice. SBP, systolic blood pressure; DBP, diastolic blood pressure; MAP, mean arterial pressure. n = 7. *P < 0.05 versus all other groups, factorial ANOVA, Holm-Sidak test. (B and C) Whole homogenates (30 μg) of kidneys isolated from A were electrophoresed (SDS-PAGE gel) and immunoblotted with anti-AT₁R (B) or anti-renin (C) antibody. Amounts of glycosylated AT₁R and renin proteins relative to actin were quantified by densitometry. The lanes for the renin blot were run on the same gel but were noncontiguous. n = 3–5. *P < 0.05 versus vehicle-treated Drd5^+/+, factorial ANOVA, Holm-Sidak test. Data are mean ± SEM.