Development of type 2 diabetes following intrauterine growth retardation in rats is associated with progressive epigenetic silencing of Pdx1
J. Clin. Invest. Jun H. Park, et al. 118:2316 doi:10.1172/JCI33655 [
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Figure 1Methylation status of proximal promoter of
Pdx1.
(
A) Map of
Pdx1. The proximal promoter of
Pdx1 is part of a more extensive CpG island. Filled circles represent CpG dinucleotides, and numbers indicate positions relative to the transcriptional start site. The position of the E-box bound by USF-1 is indicated. (
B) Methylation of CpG dinucleotides within the promoter of
Pdx1. Islets were isolated from 5 IUGR and 5 control animals at 6 months of age, and
Pdx1 DNA methylation was quantified by pyrosequencing of bisulfite-treated DNA. We did not detect methylation at any CpG of
Pdx1 in controls. The shaded bars represent data obtained and averaged from 5 IUGR animals and presented as percent methylation at each CpG site. Data are ± SEM.
