Development of type 2 diabetes following intrauterine growth retardation in rats is associated with progressive epigenetic silencing of Pdx1
J. Clin. Invest. Jun H. Park, et al. 118:2316 doi:10.1172/JCI33655 [Go to this article.]

Figure 2
Quantitative analysis of Dnmt1, Dnmt3a, and Dnmt3b bound at the Pdx1 promoter region. ChIP analysis of cross-linked chromatin from islets of IUGR and control animals at 2 weeks and 6 months of age IP with antibody to Dnmt1, Dnmt3a, and Dnmt3b. Control (Con) and IUGR samples were run on the same gel. Input DNA (In) represents PCR products without prior IP. The IgG IP showed negligible PCR product, indicating little or no IP in the absence of primary antibody. The relative amount of Dnmt1-, Dnmt3a-, and Dnmt3b-bound Pdx1 promoter was measured by genomic quantitative real-time PCR (Q-PCR) and normalized to input DNA. Data are represented as percent of control values. n = 3 experiments, data are ± SEM; *P < 0.05 versus controls.