Development of type 2 diabetes following intrauterine growth retardation in rats is associated with progressive epigenetic silencing of Pdx1
J. Clin. Invest. Jun H. Park, et al. 118:2316 doi:10.1172/JCI33655 [Go to this article.]

Figure 5
Recruitment of repressor complex at Pdx1 in IUGR islets. ChIP analysis of cross-linked chromatin from islets of IUGR and control animals at fetal day 21 (A), 2 weeks (B), and 6 months of age (C) IP with antibody to HDAC1 and mSin3A. Input DNA represents PCR products without prior IP. The IgG IP showed negligible PCR product, indicating little or no IP in the absence of primary antibody. The relative amount of HDAC1 and mSin3A bound Pdx1 promoter was measured by Q-PCR and normalized to input DNA. Data are represented as percent of control values. n = 3 experiments, data are ± SEM; *P < 0.05 versus controls.