BRAF gene duplication constitutes a mechanism of MAPK pathway activation in low-grade astrocytomas
J. Clin. Invest. Stefan Pfister, et al. 118:1739 doi:10.1172/JCI33656 [
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Figure 3Pharmacological inhibition of MAPK signaling in cell lines derived from low-grade gliomas. (
A) Proliferation of glioma cells in vitro after treatment of 4 different cell lines derived from primary low-grade gliomas with the MEK1/2 inhibitor U0126 at a concentration of 20 μM as assessed by MTT assay over a time course of 48 hours. A °II, diffuse astrocytoma; OA, oligoastrocytoma. (
B) Effective growth inhibition in all 4 cell lines can be achieved over a broad spectrum of concentrations ranging from 1 μM up to 20 μM. Medians and SDs of triplicate measurements at 48 hours are shown. (
C) Dephosphorylation of ERK1/2 is readily detectable after 30 minutes and is maintained for 48 hours after a single dose of inhibitor at a concentration of 1 μM. (
D) Maximal downregulation of CCND1 mRNA expression after treatment with MEK1/2 inhibitor U0126 at a concentration of μM was observed after 24 hours.
