STAT3 and STAT1 mediate IL-11–dependent and inflammation-associated gastric tumorigenesis in gp130 receptor mutant mice
J. Clin. Invest. Matthias Ernst, et al. 118:1727 doi:10.1172/JCI34944 [
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Figure 3Tumor suppression in
gp130Y757F/Y757FIl11ra1–/– mice coincides with reduced IL-11 expression, STAT3 activation, and target gene expression.
(
A) Immunoblot analyses were performed on lysates prepared from antral and fundic gastric tissues of 12- to 14-week-old mice using the indicated antibodies. Q-PCR expression analyses of
Socs3 (
B) in antral or fundic gastric tissue of the above mice, as well as
Il11 and putative STAT3 target genes (
C) were performed on cDNA derived from antral tissue of 14-week-old mice of the indicated genotypes. Expression data from 3–5 samples per genotype following normalization for
18S expression are shown and are presented from replicate analysis as the mean fold induction ± SD relative to expression in
gp130+/+ samples. *
P < 0.05 versus expression in
gp130+/+ samples; **
P < 0.05 versus expression in
gp130Y757F/Y757F samples.
